Molecular Combing
Genomic Vision’s AI powered molecular combing technology is at the forefront of advanced genomic analysis in bioproduction, cell & gene therapy, and drug discovery. combing is a technology that untangles the human genome and allows its direct visualization at the single molecule level.
Our studies
Our genome studies
Our molecular combing technology is a transformative tool enabling advanced structural, functional and dynamic studies of the human genome. The technique stretches a large number of genomes onto a single coverslip, facilitating the analysis of genomic variations at an unprecedented scale and providing statistically significant results for each analysis.
A new dimension for genomic DNA analysis
Our molecular combing technology allows to perform two assays: The Genetic Morse Code (GMC), and the Replication Combing Assay (RCA). GMC investigates the structural variations of the genome, whilst RCA focuses on the dynamics of DNA replication and repair. This integration opens a new horizon for genomic DNA analysis, empowering precise evaluations and improving quality control.
Advantages
Single molecule DNA studies
The process of separating and stretching DNA molecules facilitates the study of specific genomic regions. This ability enables the recognition and characterization of large-scale genomic variations, enhancing the quality control standards in bioproduction.
Preserving genomic
integrity
Our technology excels in preserving genomic integrity, by avoiding genomic DNA fragmentation. This approach allows the simultaneous study of multiple events occurring in the same genomic region, ensuring accurate and reliable results.
Constant DNA stretching factor
Our molecular combing system (MCS) consistently stretches DNA molecules at a constant rate. This allows us to precisely evaluate crucial parameters such as replication speed and inter-origin distances. Our MCS is an integral part of our high-performance, AI-powered data analysis system, facilitating accurate identification of genomic variations.
Genomic Morse
Code (GMC)
The GMC is a unique detection strategy allowing the direct visualization of large regions of interest to address structural variations and dynamic genomic events.
Applications
Replication Combing Assay (RCA)
RCA is an advanced, highly accurate tool that provides real monitoring of the spatial and temporal characteristics of genomic DNA replication at a single-molecule resolution. Under a variety of experimental conditions, hundreds of intact genomic DNA molecules are stretched simultaneously at a constant elongation rate. This allows the precise evaluation of replication speed and distances between specific events occurring on the same molecule.
APPLICATIONS
Drug Discovery
In the field of cancer treatment, conventional chemotherapies, in conjunction but also targeted therapies, utilize cytotoxic compounds that kill tumor cells by targeting their genomic DNA replication or their DNA Damage Response (DDR). RCA’s ability to monitor genome-wide replication dynamics at single-molecule resolution allows us to accurately assess the potency of these anti-cancer compounds and to better understand their mechanisms of action. Several features of genomic instability, including replication fork stalling/slowdown, restart or variations of inter-origin/termination distances, are systematically analyzed. With its RCA technology, Genomic Vision offers crucial support to pharmaceutical and biotechnology companies, de-risking and expediting the drug discovery and development process.
Research
Oncology is a highly competitive research area, driven by an increasing incidence of cancer worldwide. Over the years, DNA fiber analysis/RCA has emerged as the gold standard approach to decipher global DNA metabolism and to evaluate the replication stress induced by any genotoxic agent, such as anticancer chemotherapy compounds and radiation therapies. Genomic Vision’s unique value-added services such as experimental design guidance and automated analysis, assists researchers in gaining deep molecular insights into these intricate biological processes.