Genomic Morse Code
A unique language for a clear understanding of large DNA modifications
A proprietary detection strategy to reveal known and undiscovered structural variations
For comprehensive analysis and physical mapping of target regions on stretched DNA, we developed a unique, proprietary detection strategy, called the Genomic Morse Code (GMC). The combed DNA is hybridized with a combination of fluorescent probes of different colors and sizes, designed to recognize a selected region of interest. As a result, the DNA sequence to be analyzed is labelled with the combination of “dashes and dots”, creating a “Morse Code” specific to a target gene and its flanking regions.
The strategy underlying GMC is to use the spatial distribution of the probes to provide additional information than simply measuring just the probes. The recognition of different motifs in the Morse Code is not only based on probe size and color, but also on their order and the distances between them. The identical stretching of the DNA allows for accurate and reproducible measurements of the length of the probes as well as the gaps separating them. Any change in the observed pattern compared to the Morse Code of reference indicates the presence of a rearrangement in the target locus. Amplifications, deletions, repeats, inversions and translocations can be identified and analyzed depending on the chosen Morse Code design with no bias due to sequence content. The GMC method allows the detection of balanced rearrangements often missed by other methods and also provides information about the location and the exact number of copies found.
TECHNOLOGY
The Genomic Morse Code uses colors and spatial distribution of the probes
Size changes are directly related/indicating structural variations with no ambiguity
No confusion between the localization and variation information
- The GMC strategy allows the coverage of large loci of interest exploiting combinatorics of a few probe features
- The approach relies on probe hybridization thus any genomic region can be addressed using this approach
- MorseCodes are designed to detect the presence of Structural Variations without prior knowledge of the size, position or mutations types
- Additional specific MoseCode design can be performed to increase breakpoint resolution and decrypt complex mutation pattern
A specifically developed software is capable of detecting and analyzing all signals on a coverslip and “translating” the Genomic Morse Code into valuable information about gene modifications. Thanks to the automatic detection and the analysis of the alterations in the Morse Codes, the identification of known and also undiscovered structural variations is possible.
GMC for Clinical Research and Diagnostics
Based on the Molecular Combing approach and our Genomic Morse Code detection strategy, Genomic Vision’s GMC sets enable the rapid and accurate identification of large DNA rearrangements involved in genetic diseases and cancer.
GMC for Biomarker Discovery
A precise and targeted tool to study large scale DNA modifications involved in diseases.
