FiberProbes ® On Demand
- Hybridization-based mapping of target genes on combed DNA molecules
- Direct readout of large DNA modifications larger than 1 kb
- Detection of Structural Variations without prior knowledge of the size, position or type
- Balanced and Imbalanced rearrangements can both be detected
The Genomic Morse Code: A unique language to understand large DNA modifications.
For comprehensive analysis and physical mapping of target
regions on stretched DNA, we developed a unique, proprietary
detection strategy, called the Genomic Morse Code (GMC).
The combed DNA is hybridized with a combination of
fluorescent probes of different colors and sizes, designed to
recognize a selected region of interest. As a result, the DNA
sequence to be analyzed is labelled with the combination of
“dashes and dots”, creating a “Morse Code” specific to a
target gene and its flanking regions.
We call these custom designed probe sets FiberProbes
Reveal known and undiscovered structural variations.
The recognition of different motifs in the Morse Code is not
only based on probe size and color, but also on their order
and the distances between them. The identical stretching
of the DNA allows for accurate and reproducible measurements of the length of the probes as well as the gaps separating them.
A specifically developed software, called FiberStudio®, is
capable of detecting and analyzing all signals on a coverslip
and “translating” the Genomic Morse Code into valuable
information about gene modifications.
Any change in the observed pattern compared to the Morse Code of reference indicates the presence of a rearrangement in the target locus.
- Probes from 1 kb to 150+ kb
- Precision ~1 kb
- Possible multiplexing
- Hybridization using random primed labelled oligos
- 3 color detection